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They discovered that no normal cerevisiae or carlsbergensis strains utilised lysine whereas many other yeasts, together with wild yeasts, did so. They kept their stock cultures on malt extract agar slopes or on malt extract chalk agar within the case of Brettanomyces species. Later, Morris & Eddy1 described a stable lysine medium for the isolation and enumeration of untamed yeasts in pitching yeast.
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